Effect of intracoronary transplantation of autologous bone marrow mononuclear cells on myocardial ischemia reperfusion injury in mini-swine model / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 86-90, 2008.
Article
in Zh
| WPRIM
| ID: wpr-298739
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the therapeutic effectiveness of intracoronary transplantation of autologous bone marrow mononuclear cells (BM-MNC) on myocardial ischemia reperfusion injury in mini-swine model.</p><p><b>METHODS</b>Myocardial ischemia reperfusion injury model was established by ligating in 16 mini-swines, which were further randomized into two groups: (3.54 +/- 0.90) x 10(8) BM-MNC was intracoronarily transplanted in BM-MNC group (n = 9), and phosphate buffer saline was intracoronarily applied in the control group (n = 7). Ultrasonic cardiograhpy, hemodynamics, neovascular density, and myocardium infarction size were evaluated before and 4 weeks after transplantation.</p><p><b>RESULTS</b>In BM-MNC group, left ventricular ejection fraction (LVEF), intra-ventricular septa, lateral wall and anterior wall, cardiac output (CO) and + dp/dt(max) had no significant differences before and 4 weeks after transplantation (P > 0.05). In the control group, LVEF, intraventricular septa, lateral wall and anterior wall, CO, and + dp/dt(max) significantly decreased 4 weeks after transplantation (P < 0.05). Left ventricular end-diastolic pressure and- dp/dt(max) had no significant differences before and after cell transplantation. Capillary density was significantly larger in the BM-MNC group than in the control group [(13.39 +/- 6.96) /HP vs. (3.50 +/- 1.90) /HP]. The percentage and size of myocardial infarction was significantly lower in the BM-MNC group than in the control group.</p><p><b>CONCLUSION</b>Transplantation of BM-MNC into the myocardial ischemic reperfusion-injury area can increase capillary density and decrease infarction area, and thus remarkably improve cardiac systolic function.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Swine
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Swine, Miniature
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Therapeutics
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Myocardial Reperfusion Injury
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Random Allocation
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Bone Marrow Transplantation
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Coronary Vessels
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Myocardium
Limits:
Animals
Language:
Zh
Journal:
Acta Academiae Medicinae Sinicae
Year:
2008
Type:
Article