CD133(+) cells derived from human placenta identified as initiating cells for LTC-IC colony formation / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 125-128, 2009.
Article
in Zh
| WPRIM
| ID: wpr-302183
Responsible library:
WPRO
ABSTRACT
The aim of this study was to evaluate whether human placenta CD133(+) cells have an ability to reconstitute long-term hematopoiesis. Magnetic-activated cell sorting (MACS) was applied to enrich human placental CD133(+) cells. The isolated human placental CD133(+)cells of four different densities were established by limiting-dilution assay and primary fetal bone marrow stromal cells separated from bone marrow as feeder layer cells were co-cultured in long-term culture system so as to observe the incidence of long-term culture initiating-cells (LTC-IC) and their ability of proliferation and differentiation.The results showed that human placenta derived CD133(+) cells contained LTC-IC with frequency of 1/645 which have an ability to proliferate and differentiate into granulocyte/macrophage colony-forming units (CFU-GM) and mixed colony-forming units (CFU-Mix). In all LTC-IC positive wells, 71.43% form only CFU-GM and 28.57% display both CFU-GM and CFU-Mix formation. It is concluded that human placental CD133(+) cells possess LTC-IC with colony-forming capacity of hematopoietic early progenitor cells.
Full text:
1
Index:
WPRIM
Main subject:
Peptides
/
Placenta
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Hematopoietic Stem Cells
/
Glycoproteins
/
Antigens, CD
/
Cell Differentiation
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Cell Separation
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Colony-Forming Units Assay
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Cell Culture Techniques
/
Cell Biology
Limits:
Female
/
Humans
/
Pregnancy
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article