Effect of overexpression of nicotinic acid phosphoribosyl transferase on succinic acid production in Escherichia coli NZN111 / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1438-1447, 2011.
Article
in Zh
| WPRIM
| ID: wpr-304559
Responsible library:
WPRO
ABSTRACT
Escherichia coli strain NZN111 is a promising candidate for the fermentative production of succinate. However, because lactate dehydrogenase and pyruvate formate lyase were inactivated in NZN111, this strain had an unbalanced NADH/NAD+ ratio and could not use glucose under anaerobic conditions. In this study, a recombinant strain E. coli NZN111/pTrc99a-pncB was constructed to overexpress the nicotinic acid phosphoribosyl transferase gene (pncB). Under anaerobic conditions with the addition of 0.5 mmol/L nicotinic acid and 0.3 mmol/L isopropyl beta-D-thiogalactopyranoside (IPTG), the specific nicotinic acid phosphoribosyl transferase (NAPRTase, EC 2.4.2.11) activity in the recombinant strain was 11-fold higher than that in E. coli NZN111, the concentration of NAD(H) was increased by 3.85-fold, especially the concentration of NAD+ was increased by 5.17-fold and NADH/NAD+ was decreased from 0.640 to 0.125. The recombinant strain regained the capability of growth and glucose utilization under anaerobic conditions.
Full text:
1
Index:
WPRIM
Main subject:
Acetyltransferases
/
Classification
/
Succinic Acid
/
Genetic Enhancement
/
Escherichia coli
/
Nicotinamide Phosphoribosyltransferase
/
Fermentation
/
Genetics
/
Glucose
/
Anaerobiosis
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2011
Type:
Article