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Expression and detection of recombinant directing toxin SL120 in Pichia pastoris / 生物工程学报
Chinese Journal of Biotechnology ; (12): 473-477, 2005.
Article in Chinese | WPRIM | ID: wpr-305248
ABSTRACT
Anti-HIV-1 gp120 single chain antibody(scFv) gene and staphylococcus extoxin A(SEA) gene were inserted into vector pPIC9K. The recombinant plasmid was integrated into Pichia pastoris by electroporation. High level expression was performed by determining the Muts phenotype and screening muti-copy integrants. The recombinant protein was about 57kD and the production was 50.1 mg/L. It was shown that the two kinds of protein affected the conformation of each other by antibody affinity assay, but the recombinant targeting toxins could highly mediate CTLs to kill HIV-1 target cells.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pichia / Recombinant Proteins / Immunoglobulin Variable Region / HIV Envelope Protein gp120 / HIV-1 / Enterotoxins / Allergy and Immunology / Genetic Vectors / Genetics / Metabolism Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pichia / Recombinant Proteins / Immunoglobulin Variable Region / HIV Envelope Protein gp120 / HIV-1 / Enterotoxins / Allergy and Immunology / Genetic Vectors / Genetics / Metabolism Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2005 Type: Article