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Effects of mercury on the structure and activity of BLM642-1290 recombinant helicase / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 47-55, 2011.
Article in English | WPRIM | ID: wpr-306892
ABSTRACT
<p><b>OBJECTIVE</b>Bloom's syndrome is an autosomal recessive disorder characterized by genomic instability and a predisposition to many cancers. Mutations of the BLM gene (encoding a BLM helicase) may form a structure of the etiology of this disease. As a global pollutant, mercury poses a major threat to human health. The current study was conducted to elucidate the effects of Hg(2+) on the structure and activity of BLM642-1290 recombinant helicase, and to further explore the molecular mechanisms of mercury toxicity to the DNA helicase.</p><p><b>METHODS</b>The effects of Hg(2+) on biological activity and structure of BLM642-1290 recombinant helicase were determined by fluorescence polarized, ultraviolet spectroscopic, and free-phosphorus assay technologies, respectively.</p><p><b>RESULTS</b>The helicase activity, the DNA-binding activity, and the ATPase activity of BLM642-1290 recombinant helicase were inhibited by Hg(2+) treatment. The LMCT (ligand-to-metal charge transition) peaks of the helicase were enhanced with the increase of the Hg(2+) level. The LMCT peaks of the same concentration of helicase gradually increased over time.</p><p><b>CONCLUSION</b>The biological activity of BLM642-1290 recombinant helicase is inhibited by Hg(2+) treatment. The conformation of the helicase is significantly altered by Hg(2+). There exist two binding sites between Hg(2+) and the helicase, which are located in the amino acid residues 1063-1066 and 940-944 of the helicase, respectively.</p>
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Protein Conformation / Spectrophotometry, Ultraviolet / Structure-Activity Relationship / Recombinant Proteins / Base Sequence / Chemistry / Adenosine Triphosphatases / DNA Primers / Toxicity / RecQ Helicases Limits: Humans Language: English Journal: Biomedical and Environmental Sciences Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Protein Conformation / Spectrophotometry, Ultraviolet / Structure-Activity Relationship / Recombinant Proteins / Base Sequence / Chemistry / Adenosine Triphosphatases / DNA Primers / Toxicity / RecQ Helicases Limits: Humans Language: English Journal: Biomedical and Environmental Sciences Year: 2011 Type: Article