Regulation of EGF-receptor expression by TGFalpha in human prostate androgen-unresponsive cancer cells / 中华男科学杂志
National Journal of Andrology ; (12): 595-597, 2004.
Article in Chinese | WPRIM | ID: wpr-308292
Responsible library: WPRO
ABSTRACT<p><b>OBJECTIVE</b>To elucidate the regulation of epidermal growth factor receptor (EGFR) expression by transforming growth factor (TGFalpha) and epidermal growth factor (EGF) in human prostate androgen-unresponsive cancer cells.</p><p><b>METHODS</b>EGFR mRNA expression and its protein level were measured by means of RT-PCR and Western blot respectively in human prostate cancer androgen-unresponsive cell lines, ARCaP and PC3, all treated with exogenous TGFalpha.</p><p><b>RESULTS</b>In the TGFalpha group, the levels of EGFR mRNA were 5.01 0.45 and 9.05 0.63 in PC3 and ARCaP respectively, significantly higher than in the control group (P < 0.05). The level of EGFR protein in PC3 treated with TGFalpha was 2.28 0.53, higher than in the control group (P < 0.05); however, the level of EGFR protein in ARCaP treated with TGFalpha was only 1.24 0.22, not different from the control (P > 0.05).</p><p><b>CONCLUSION</b>TGFalpha/EGF-EGFR pathway serves as a key growth regulator in prostate cancer. TGFalpha, but not EGF, preferentially maintains an autocrine loop in human androgen-unresponsive prostate cancer.</p>
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Prostatic Neoplasms / Humans / Male / RNA, Messenger / Blotting, Western / Transforming Growth Factor alpha / Reverse Transcriptase Polymerase Chain Reaction / Cell Line, Tumor Language: Chinese Journal: National Journal of Andrology Year: 2004 Type: Article