Assessment of heme oxygenase-1 (HO-1) activity in the cavernous tissues of sildenafil citrate-treated rats / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
; (6): 377-381, 2007.
Article
in En
| WPRIM
| ID: wpr-310500
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To assess heme oxygenase-1 (HO-1) activity in the cavernous tissue of sildenafil citrate-treated rats.</p><p><b>METHODS</b>One hundred and ninety-two Sprague-Dawley male rats, divided into four equal groups, were investigated. Group 1, the control group, received regular animal chow; group 2 received sildenafil citrate by intragastric tube; group 3 received sildenafil and HO inhibitor (zinc protoporphyrin, ZnPP); and group 4 received sildenafil and nitric oxide synthase (NOS) inhibitor L-nitroarginine methyl ester (L-NAME). Twelve rats from each group were killed after 0.5 h, 1 h, 2 h and 3 h of drug administration. Then HO-1 activity, cGMP levels and NOS enzymatic activity in the cavernous tissues were estimated.</p><p><b>RESULTS</b>In cavernous tissue, HO-1 activity, NOS enzymatic activity and cGMP concentration increased significantly in sildenafil-treated rats compared to other groups throughout the experiment. Rats receiving either HO or NOS inhibitors showed a significant decrease in these parameters. HO-1 cavernous tissue activity and NOS enzymatic activity demonstrated a positive significant correlation with cGMP levels (r = 0.646, r = 0.612 respectively; P < 0.001).</p><p><b>CONCLUSION</b>The actions of PDE5 inhibitor sildenafil citrate in the cavernous tissue are partly mediated through the interdependent relationship between both HO-1 and NOS activities.</p>
Full text:
1
Index:
WPRIM
Main subject:
Penis
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Pharmacology
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Piperazines
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Protoporphyrins
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Purines
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Sulfones
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Vasodilator Agents
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Administration, Oral
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Rats, Sprague-Dawley
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Cyclic GMP
Limits:
Animals
Language:
En
Journal:
Asian Journal of Andrology
Year:
2007
Type:
Article