Study on fluorescence sequencing typing technology identification of raw materials in liuwei dihuang pill / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3695-3700, 2014.
Article
in Zh
| WPRIM
| ID: wpr-311006
Responsible library:
WPRO
ABSTRACT
In this paper, Liuwei Dihuang pill was used to study the identification of Chinese patent medicine by fluorescence sequencing typing technology. The DNA of Paeonia suffruticosa was used as template to amplify by five pair of FAM fluorescence labeling primers. Then, the amplified products were sequenced. The sequencing results were analyzed by GeneMarker V1.80 to screen the best fluorescence labeling primers. As a result, psbA-trnH fluorescence labeling primer was used to identify the raw materials of Liuwei Dihuang pill. The results showed that three kinds of raw plant medicinal materials in Liuwei Dihuang pill were able to be correctly identified by psbA-trnH fluorescence labeling primer. The fluorescence sequencing typing technology can stably and accurately distinguish raw medicinal materials in Chinese patent medicine.
Full text:
1
Index:
WPRIM
Main subject:
Plants, Medicinal
/
Quality Control
/
Reference Standards
/
Staining and Labeling
/
Drugs, Chinese Herbal
/
Chemistry
/
Polymerase Chain Reaction
/
DNA Primers
/
DNA, Plant
/
Fluorescent Dyes
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article