Change of structural maintenance of chromosome (SMC)1, SMC3, Separase and Securin expression in BEAS-2B malignant transformation cell induced by coal tar pitch smoke extracts / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 776-780, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-313540
ABSTRACT
<p><b>OBJECTIVE</b>to study the role of structural maintenance of chromosome (SMC)1, SMC3, Separase and Securin in tumorgenesis that contact with coal tar pitch.</p><p><b>METHODS</b>the BEAS-2B cells was induced by coal tar pitch smoke extracts to form malignant transformation cell model in vitro. The gene expression levels of mRNA were assessed by real-time quantitative RT-PCR, and the protein expression variation were determined by cell culture overslip of immunohistochemical methods.</p><p><b>RESULTS</b>in malignant transformation cells, the mRNA and the protein expression level of SMC1 gene was not statistically significantly different compared with the BEAS-2B group and DMSO group (P > 0.05); SMC3 and Separase was increased and Securin was decreased (P < 0.05), while the difference between other two control groups was not significant (P > 0.05).</p><p><b>CONCLUSIONS</b>the up expression level of SMC3 and Separase and the down expression level of Securin are involved in the process that evolves into malignant transformation in bronchial epithelial cells BEAS-2B induced by coal tar pitch smoke extracts.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Endopeptidases
/
Chondroitin Sulfate Proteoglycans
/
Sister Chromatid Exchange
/
Smoke
/
Bronchi
/
Cell Line, Transformed
/
Chromosomal Proteins, Non-Histone
/
Cell Line
/
Coal Tar
/
Cell Cycle Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2010
Type:
Article
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