Determination of scandoside methyl ester in Hedyotis chrysotricha by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop an HPLC method for determination of scandoside methyl ester in Hedyotis chrysotricha.</p><p><b>METHOD</b>The determination was carried out on an HC-C18 column elnted with acetonitrile-water (7:93) as mobile phase, and the detection wavelength was set at 238 nm.</p><p><b>RESULT</b>There is a good linearity in the range 22.08-552 mg L(-1) of scandoside methyl ester and the average recovery is 97.7% (n = 6), RSD 0.72%.</p><p><b>CONCLUSION</b>This method is convenient, quick, and is applicable to the quality control of H. chrysotricha.</p>