Rapidly detect and distinguish between norovirus G I and G II type with a pair of primers / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 379-381, 2013.
Article
in Zh
| WPRIM
| ID: wpr-318014
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>The purpose of this study was to develop RT- PCR assay for Rapidly detect and distinguish between Norovirus genogroup I and genogroup II with a pair of primers.</p><p><b>METHODS</b>A pairs of primers specific to capsid prote in ORF2 gene of G I and G II Norovirus were dsigned according to the published complete genome sequence, with which the RNA of Norovirus was extracted and RT-PCR amplification. The sensitivity, specificity of the RT- PCR assay was estimated and apply it to the detection of Norovirus in clinical specimens.</p><p><b>RESULTS</b>The results showed that the assay possessed high specificity for Norovirus detection and without any evident cross-reaction with other viruses, including rotavirus, enteric adenovirus and hepatitis A virus. The detection limit of RT-PCR assay for Norovirus G I and G II were up to 100 pg/ml and 10 pg/ml respectively.</p><p><b>CONCLUSION</b>The RT- PCR assay provide rapid and sensitive detection of Norovirus G I and G II and should prove to be useful for Norovirus diagnosis in the outbreaks of acute gastroenteritis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Virology
/
Classification
/
DNA Primers
/
Caliciviridae Infections
/
Reverse Transcriptase Polymerase Chain Reaction
/
Norovirus
/
Diagnosis
/
Gastroenteritis
/
Genetics
/
Methods
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2013
Type:
Article