Experimental study of periosteal osteoblasts adhesion to artificial bone scaffolds based on rapid prototype / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 248-251, 2004.
Article
in Zh
| WPRIM
| ID: wpr-319006
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the biocompatibility of bone engineering scaffolds designed and fabricated by CAD and Rapid Prototyping techniques.</p><p><b>METHODS</b>Infant rat calvarias osteoblasts were isolated and expanded in vitro and the cells (2nd passage) were seeded onto scaffolds with porosity 80%, 90%, 95% at a density of 2.06 x 10(9)/L. Cell adhesion number and morphology were measured with SEM after 4 days, 10 days co-culture.</p><p><b>RESULTS</b>(1) The osteoblasts' adhesion amounts increased with culture time in three porosity group (P < 0.05), but the increase were different among three groups, 80% group was 0.35 x 10(5), 90% group was 2.84 x 10(5); (2) Through SEM observations, it showed that osteoblasts adhered to all scaffolds well.</p><p><b>CONCLUSION</b>The scaffolds designed and fabricated by CAD and rapid prototyping own a good cellular biocompatibility. The results suggest the feasibility of using such scaffold fabricating method for bone tissue engineering research and clinical therapy.</p>
Full text:
1
Index:
WPRIM
Main subject:
Osteoblasts
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Cell Adhesion
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Cells, Cultured
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Rats, Sprague-Dawley
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Bone Substitutes
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Cell Biology
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Tissue Engineering
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Methods
Limits:
Animals
Language:
Zh
Journal:
West China Journal of Stomatology
Year:
2004
Type:
Article