Construction of the recombinant adenovirus vector bearing dual-survivin shRNA / 中华男科学杂志
National Journal of Andrology
; (12): 324-327, 2008.
Article
in Zh
| WPRIM
| ID: wpr-319260
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct a recombinant adenovirus vector bearing dual-survivin short hairpin RNA (shRNA).</p><p><b>METHODS</b>Dual-survivin shRNA was designed and synthesized respectively, both inserted into adenovirus DNA. The recombinant adenovirus vector was confirmed via both sequencing and restriction digestion analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenoviruses.</p><p><b>RESULTS</b>The recombinant adenovirus vector was constructed and the target sequence was obtained.</p><p><b>CONCLUSION</b>The construction of the recombinant adenovirus vector provides a basis for the research of potential gene therapy for prostate cancer.</p>
Full text:
1
Index:
WPRIM
Main subject:
Recombinant Fusion Proteins
/
Transfection
/
DNA Restriction Enzymes
/
Cell Line
/
Adenoviridae
/
Polymerase Chain Reaction
/
RNA, Small Interfering
/
Green Fluorescent Proteins
/
Inhibitor of Apoptosis Proteins
/
Genetic Vectors
Limits:
Humans
Language:
Zh
Journal:
National Journal of Andrology
Year:
2008
Type:
Article