Analysis of monosaccharide compositions in polysaccharides from exopleura of Ginkgo biloba / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 262-266, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-319617
ABSTRACT
A high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5 -pyrazolone (PMP) has been established for determination of 6 kinds of monosaccharides simultaneously. A special Agilent HC-C18 column (4. 6 mm x 250 mm, 5 microm), optimized for the separation of PMP derivatives, was used at ambient temperature of 40 degrees C. The PMP derivatives elution was performed with a mixture of 0.1 mol x L(-1) phosphate buffer (pH 6. 8) and acetonitrile in a ratio of 84 16 at a flow rate of 1 mL x min(-1), and UV absorbance of the effluent was monitored at 245 nm. The results showed that the polysaccharides from exopleura of Ginkgo biloba were acidic heteropolysaccharides mainly containing mannose, rhamnose, D-galacturonic acid, glucose, galactose, arabinose, with the molar ratio of 0.032 0.14 0.296 0.4030.106 0.046.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polysaccharides
/
Chemistry
/
Ginkgo biloba
/
Plant Components, Aerial
/
Hydrolysis
/
Monosaccharides
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article
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