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Intestinal absorption of pulchinenosides from Pulsatilla chinensis in rats / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 543-549, 2015.
Article in Chinese | WPRIM | ID: wpr-330239
ABSTRACT
HPLC-ELSD was applied to explore the absorption mechanism of pulchinenosides (B3, BD, B7, B10, B11) in rats. The experimental results showed that the absorption rate constant, Ka value (B3, BD) and Permeability coefficient, Peff value (B3, B7) displayed significant difference (P <0.05) in various intestinal segments, The Ka value and Peff value of PRS was different from each other with the highest absorption in duodenum (duodenum > jejunum > colon > ileum); The PRS displayed excessive satuation as the concentration increased over 0.05-2.5 g · L(-1). There were no obvious linear correlations between Peff values and concentrations in duodenum (0.6007 ≤ R2 ≤ 0.7727); Ka and Peff value declined when the PRS was perfused with P-glycoprotein promoter digoxin, on the other hand, inclined when perfused with P-glycoprotein inhibitor verapamil with significant difference among PRS B3, BD, B7, B11 (P <0.05). All the above results demonstrated that B3, BD, B7 were greatly influenced by absorption sites, duodenum was the main absorption site; PRS didn't entirely transported in a concentration dependent manner, and the transporter-protein involved the transportation, so the intestinal absorption of the five pulchinenosides was not entirely passive diffusion; and PRS might be the substrates of P-glycoprotein.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Oleanolic Acid / Physiology / Saponins / Pharmacokinetics / Chemistry / Rats, Wistar / ATP Binding Cassette Transporter, Subfamily B, Member 1 / Pulsatilla / Intestinal Absorption Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Oleanolic Acid / Physiology / Saponins / Pharmacokinetics / Chemistry / Rats, Wistar / ATP Binding Cassette Transporter, Subfamily B, Member 1 / Pulsatilla / Intestinal Absorption Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2015 Type: Article