Induction of apoptosis in human hepatoma cell line SMMC7721 by Newcastle disease virus HN gene / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 279-282, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-331171
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanisms of apoptosis induced in human hepatoma cell line SMMC7721 by plasmid pVHN constructed with Newcastle disease virus (NDV) HN gene.</p><p><b>METHODS</b>Twenty-four h after transfection with liposome-plasmid pVHN complexes in vitro, the mortality rate of SMMC7721 cells was determined by MTT staining and flow cytometry (FCM) with PI staining. The alteration of mitochondrial trans-membrane potential of the cells was detected by FCM with rhodamine 123 staining. Cell genomic DNA was detected by agarose electrophoresis. The activation of caspase-3 was assayed by its substrate color reaction.</p><p><b>RESULTS</b>Significant apoptosis was induced by transfection with plasmid pVHN into the cells for 24 h and the mortality rate was 50.0% (the mortality rate of control group was 5.2%). Genomic DNA was fragmented and mitochondrial trans-membrane potential was decreased, but caspase-3 activity increased.</p><p><b>CONCLUSION</b>Significant apoptosis in SMMC7721 cells can be induced by NDV HN gene. Apoptosis may be resulted from the decrease of mitochondrial trans-membrane potential and activation of Caspase-3.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Newcastle disease virus
/
Transfection
/
HN Protein
/
Apoptosis
/
Carcinoma, Hepatocellular
/
Cancer Vaccines
/
Vaccines, DNA
/
Cell Line, Tumor
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2005
Type:
Article
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