Study of lipopolysaccharide antagonizing effect of DPR-2 in vitro / 中华烧伤杂志
Chinese Journal of Burns
; (6): 104-107, 2007.
Article
in Zh
| WPRIM
| ID: wpr-331515
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the lipopolysaccharide (LPS) antagonizing biological activity of densefruit pattany root-bark extract (DPR-2) in vitro.</p><p><b>METHODS</b>The effect of DPR-2 in neutralizing LPS (0.1 microg/L) was detected by kinetic turbidimetric limulus test. The effect of different concentrations of DPR-2 (0,8.0,16.0,32.0,64.0 mg/L) on binding of FITC-conjugated LPS (FITC-LPS,100.0 microg/L) to murine RAW264.7 cells was analyzed with laser scanning confocal microscopy. The expression of TNF-alpha and IL-6 mRNA in RAW264.7 cells after exposure to LPS (100.0 microg/L) were determined by real-time RT-PCR.</p><p><b>RESULTS</b>DPR-2 could neutralize LPS (P < 0.05 or P < 0.01), and inhibit the binding of FITC-LPS to RAW264.7 cells in a dose-dependent manner when the concentration of DPR-2 was above 16.0 mg/L. Furthermore, DPR-2 could markedly inhibit the expression of TNF-alpha and IL-6 mRNA in LPS-stimulated murine RAW264.7 cells.</p><p><b>CONCLUSION</b>DPR-2 exhibit an anti-LPS effect in vitro, which may be related to its capacity to neutralize LPS and inhibit binding of LPS for its receptors.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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In Vitro Techniques
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Drugs, Chinese Herbal
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Plant Extracts
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Monocytes
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Cell Line
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Lipopolysaccharides
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Endotoxins
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Limulus Test
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Metabolism
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Burns
Year:
2007
Type:
Article