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Effects of β-elemene on proliferation and apoptosis of SGC7901 gastric cancer cells in vitro and the underlying mechanisms / 南方医科大学学报
Journal of Southern Medical University ; (12): 1234-1238, 2015.
Article in Chinese | WPRIM | ID: wpr-333649
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of β-elemene in suppressing the proliferation and apoptosis of SGC7901 gastric cancer cells in vitro and explore the underlying mechanisms.</p><p><b>METHODS</b>Using MTT assay, flow cytometry, and clonogenic survival assay, we assessed the effects of β-elemene on the viability, apoptosis, cell cycle distribution, and clonogenic survival of gastric cancer SGC7901 cells and gastric mucosal epithelial GES-1 cells. Western blotting was employed to determine the changes in the protein expression profiles in SGC7901 cells in response to β-elemene treatment.</p><p><b>RESULTS</b>β-elemene significantly suppressed the cell viability and increased the apoptosis of SGC7901 cells, and these effects were less obvious in GES-1 cells. β-elemene decreased clonogenic survival of SGC7901 cells, increased the proportion of G2/M phase cells, decreased the expression of Bcl-2, and increased the expression of Bax and cleaved caspase-3. β-elemene did not obviously affect the expression of total p21-activated protein kinase 1 (Pak1) but decreased the level of phospho-Pak1 (Thr423) and phospho-ERK1/2 (Thr202/Tyr204) in SGC7901 cells.</p><p><b>CONCLUSION</b>β-elemene inhibits the proliferation and induces apoptosis of gastric cancer cells possibly by inhibiting Pak1/ERK signaling and regulating apoptosis-associated proteins such as Bcl-2 and Bax.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Sesquiterpenes / Stomach Neoplasms / Signal Transduction / Cell Cycle / Cell Division / Cell Survival / Apoptosis / Cell Line, Tumor Limits: Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Sesquiterpenes / Stomach Neoplasms / Signal Transduction / Cell Cycle / Cell Division / Cell Survival / Apoptosis / Cell Line, Tumor Limits: Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2015 Type: Article