Design and activity analysis of chimeric epidermal growth factor fusion vaccine E5T-mSEA / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 357-362, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-336219
ABSTRACT
Epidermal growth factor receptor (EGFR) and its ligands (EGF and TGFalpha) are over-expressed in a variety of tumors. Immunization EGF-carrier protein inhibits tumor growth through abrogating binding of EGF to EGFR. Here, a chimeric protein of EGF and TGFalpha (E5T) was genetically fused to Staphylococcal enterotoxin A (SEA), a bacterial superantigenic protein which promotes humoral B cell response through enhancement of Ag-specific CD4 T cells activity. The resulted fusion proteins were expressed in Escherichia coli and purified though metal chelating affinity chromatography. Immunization of E5T-mSEA fusion protein in mice induced production of high titers antibodies, which recognize both EGF and TGFalpha. Anti- E5T-mSEA serum at dilution of 110 significantly inhibited growth of A431 cell lines but had little effect on 293T cell lines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
Molecular Sequence Data
/
Random Allocation
/
Transforming Growth Factor alpha
/
Amino Acid Sequence
/
Immunization
/
Cancer Vaccines
/
Cell Line, Tumor
/
Enterotoxins
/
Allergy and Immunology
Type of study:
Controlled clinical trial
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2010
Type:
Article
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