Isolation, purification of collagen from soft-shelled turtle calipash for application in biomaterial / 生物工程学报
Chinese Journal of Biotechnology
; (12): 819-830, 2016.
Article
in Zh
| WPRIM
| ID: wpr-337419
Responsible library:
WPRO
ABSTRACT
Recently, research on collagen attracts more interests due to its good biological compatibility. The present study attempted to establish a fast and efficient method to purify collagen from soft-shelled turtle and to explore its application in biological materials. The structure and type of collagen fiber in calipash were determined by van Gieson staining and Picrosirius red staining, which could contribute to the isolation of collagen from soft-shelled turtle Calipash (STCC). Collagen fibers were in high content and the main collagen fiber was type I in STCC. The crude STCC solution was purified by dialysis with different cut-off molecular weight. SDS-PAGE demonstrated that the best purification was in applying 100 kDa dialysis bags after 48 h. The water absorbing capacity and holding capacity of STCC were up to 12.06 g/g and 98.21%, respectively. STCC can be degraded by collagenase in vitro entirely after 72 h. The hemolysis, skin sensitization, hemostatic and wound healing of STCC were determined by using SD rat model, and the collagen cross-linked by glutaric dialdehyde was set as a comparison. STCC and STCC cross-linked did not result in destructed red blood cell, inflamed and sensitized skin. Both materials exhibited good hemostatic effect. Thus, STCC improved the wound healing efficiently. This study implies a potential of STCC in the field of biomaterial.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Skin
/
Turtles
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Wound Healing
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Biocompatible Materials
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Biotechnology
/
Collagen
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Rats, Sprague-Dawley
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Electrophoresis, Polyacrylamide Gel
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Molecular Weight
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2016
Type:
Article