Effects of Yikunning on the apoptotic rate and expression of caspase-3 in rat ovaries during perimenopausal period / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 318-321, 2010.
Article
in Zh
| WPRIM
| ID: wpr-340162
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Yikunning (compound of Chinese traditional Medicine, YKN) on the apoptotic rate and expression of caspase-3 in rat ovaries during perimenopausal period.</p><p><b>METHODS</b>Thirty female Wistar rats during perimenopausal period were selected by unforced aging. Then the rats were divided into 3 groups randomly: YKN group, livial control group and aged control group. Ten young female rats were selected as young control group. Intragastric administrations were conducted for 4 weeks once daily continuously. The apoptotic rate in rat ovaries were detected by TUNEL. The expression of caspase-3 mRNA and protein in rat ovaries were detected by RT-PCR and Western blot, respectively.</p><p><b>RESULTS</b>The apoptotic rate in rat ovaries in YKN group was lower than that in aged control group, which showed difference between them (P < 0.01). The levels of caspase-3 mRNA and protein in rat ovaries in YKN group were lower than those in aged control group, which showed differences among them (P < 0.01).</p><p><b>CONCLUSION</b>YKN can decrease the apoptotic rate and down-regulate the expression of caspase-3 mRNA and protein in rat ovaries of during perimenopausal period. It may be one of the molecular mechanisms of YKN postponed the ovarian failure and cured perimenopausal syndrome.</p>
Full text:
1
Index:
WPRIM
Main subject:
Ovary
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Pharmacology
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Drugs, Chinese Herbal
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Rats, Wistar
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Apoptosis
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Cell Biology
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Perimenopause
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Caspase 3
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Metabolism
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Applied Physiology
Year:
2010
Type:
Article