Construction and identification of plasmid vector encoding two survivin shRNA / 中华男科学杂志
National Journal of Andrology
; (12): 512-515, 2006.
Article
in Zh
| WPRIM
| ID: wpr-343585
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct and identify the eukaryotic expression plasmids encoding two short hairpin RNA (shRNA) of survivin for the purpose of paving the way for the studies of targeted gene therapy for prostatic carcinoma (PCa).</p><p><b>METHODS</b>Two shRNA of survivin were designed and synthesized respectively, and then both were cloned into plasmids. Finally, the recombinant plasmids were confirmed by sequencing and agarose gel electrophoresis after restriction digestion.</p><p><b>RESULTS</b>The recombinant plasmids encoding two survivin shRNA were constructed and the aim sequence obtained.</p><p><b>CONCLUSION</b>Successful construction of the recombinant provides a sound basis for the research of targeted gene therapy for PCa.</p>
Full text:
1
Index:
WPRIM
Main subject:
Plasmids
/
Transfection
/
Cloning, Molecular
/
RNA, Small Interfering
/
RNA Interference
/
Green Fluorescent Proteins
/
Inhibitor of Apoptosis Proteins
/
Genetic Vectors
/
Genetics
/
Microtubule-Associated Proteins
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
National Journal of Andrology
Year:
2006
Type:
Article