Your browser doesn't support javascript.
loading
Development of radioimmunoassay for human sperm protein 17 / 中华男科学杂志
National Journal of Andrology ; (12): 510-515, 2006.
Article in Chinese | WPRIM | ID: wpr-343586
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method of radioimmunoassay for human sperm protein 17(Sp17) determination.</p><p><b>METHODS</b>Anti-recombinant human Sp17 antibody was prepared, the labeling of 125I-rhSp17 performed by chloramine T method, and radioimmunoassay of Sp17 developed.</p><p><b>RESULTS</b>The assay range was 3.3 to approximately 800 microg/L, the sensitivity was 2.0 microg/L, and the intra- and inter-assay coefficients of variation (CV) were 7.5% to approximately 9.8% and 8.2% to approximately 13.2%, respectively. The serum Sp17 level in normal subjects was (15.60 +/- 7.66) microg/L (n = 59).</p><p><b>CONCLUSION</b>This radioimmunoassay of Sp17 fulfills the reasonable requirements of clinical routine and scientific studies in terms of specificity, sensitivity and practicability. Measurement of Sp17 concentration is useful for assessing its native distribution and aberrant expression.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Blood / Recombinant Proteins / Radioimmunoassay / Carrier Proteins / Reproducibility of Results / Sensitivity and Specificity / Allergy and Immunology / Methods / Antigens, Surface Type of study: Diagnostic study Limits: Animals / Female / Humans Language: Chinese Journal: National Journal of Andrology Year: 2006 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Blood / Recombinant Proteins / Radioimmunoassay / Carrier Proteins / Reproducibility of Results / Sensitivity and Specificity / Allergy and Immunology / Methods / Antigens, Surface Type of study: Diagnostic study Limits: Animals / Female / Humans Language: Chinese Journal: National Journal of Andrology Year: 2006 Type: Article