Research progress in the third-generation genomic editing technology - CRISPR/Cas9 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 713-716, 2016.
Article
in Zh
| WPRIM
| ID: wpr-345373
Responsible library:
WPRO
ABSTRACT
CRISPR/Cas9 technology originated from type II CRISPR/Cas system, which is widely found in bacteria and equips them with acquired immunity against viruses and plasmids. CRISPR-associated protein Cas9 is a RNA-guided endonuclease, which can efficiently introduce double-strand breaks at specific sites and activate homologous recombination and/or non-homologous end joining mechanism for the repair of impaired DNA. Features such as easy-to-use, cost-effectiveness, multiple targeting ability have made it the third-generation genomic engineering tool following ZFNs and TALENs. Here the history of discovery and molecular mechanism of the CRISPR/Cas9 technology are reviewed. The rapid advance in its various applications, especially for the treatment of human genetic disorders, as well as some concomitant problems are discussed.
Full text:
1
Index:
WPRIM
Main subject:
Genome, Human
/
Reproducibility of Results
/
Biomedical Research
/
CRISPR-Cas Systems
/
Gene Editing
/
Genetics
/
Methods
/
Models, Genetic
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2016
Type:
Article