Identification of corium stomachium galli by molecular taxonomy / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a convenient and accurate method of DNA molecular marker for the identification of corium stomachium galli.</p><p><b>METHOD</b>Cytb mtDNA sequences of Gallus gallus domestica and three other species of poultry were downloaded from Genbank. Species-specific PCR primers were designed according to the differential DNA fragments of the cytb genes. PCR tests were performed with the DNAs extracted from G. gallus domestica, three other poultry species and domestic mammal animals.</p><p><b>RESULT</b>The specific primers of G. gallus domestica could only amplify the cytb mtDNA of G. gallus domestica.</p><p><b>CONCLUSION</b>The primers are specific to G. gallus domestica mtDNA and can used to discern Corium stomachium from the false medicine.</p>