Serotypes and ampicillin resistance of Haemophilus influenzae isolates from children with respiratory infection in Hangzhou / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 217-220, 2009.
Article
in Zh
| WPRIM
| ID: wpr-347956
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the serotypes distribution and ampicillin resistance of Haemophilus influenzae isolates from children with respiratory infection in Hangzhou.</p><p><b>METHODS</b>Haemophilus influenzae strains were identified with V factor and X factor tests. Serotypes were determined with the slide agglutination method. Nitrocefin test was used to detect beta-lactamase. The sensitivities of ampicillin to Haemophilus influenzae were determined with the Kirby-Bauer diffusion method and the E-test method.</p><p><b>RESULTS</b>One hundred and fifty-two Haemophilus influenzae isolates, 108 from boys and 44 from girls, were identified between December 2006 and July 2007. Of the 152 isolates, 148 (97.4%) were untypable, only 4 (2.6%) were typable, including type a, type d, type e and type f (n=1 each type). Haemophilus influenzae type b and c strain was not found. Thirty-four isolates (22.4%) were beta-lactamase-positive. One hundred and thirteen isolates (74.3%) were susceptible to ampicillin, while 34 isolates (22.4%) were resistant to ampicillin.</p><p><b>CONCLUSIONS</b>Untypable strains were the most common in Haemophilus influenzae isolates from children with respiratory infection in Hangzhou. The isolates of Haemophilus influenzae kept susceptibity to ampicillin to a certain extent.</p>
Full text:
1
Index:
WPRIM
Main subject:
Respiratory Tract Infections
/
Beta-Lactamases
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Ampicillin Resistance
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Microbial Sensitivity Tests
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Serotyping
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Haemophilus influenzae
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Classification
/
Microbiology
Type of study:
Prognostic_studies
Limits:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
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Newborn
Language:
Zh
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2009
Type:
Article