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Expression of USP15, TβR-I and Smad7 in psoriasis / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 415-419, 2014.
Article in English | WPRIM | ID: wpr-351061
ABSTRACT
The deubiquitinating enzyme ubiquitin specific peptidase 15 (USP15) is regarded as a regulator of TGFβ signaling pathway. This process depends on Smad7, the inhibitory factor of the TGFβ signal, and type I TGFβ receptor (TβR-I), one of the receptors of TGFβ. The expression level of USP15 seems to play vital roles in the pathogenesis of many neoplasms, but so far there has been no report about USP15 in psoriasis. In this study, immunohistochemical staining of USP15, TβR-I and Smad7 was performed in 30 paraffin-embedded psoriasis specimens and 10 normal specimens to investigate the expression of USP15, TβR-I and Smad7 in psoriasis and to explore the relevance among them. And USP15 small interfering RNA (USP15 siRNA) was used to transfect Hacat cells to detect the mRNA expression of TβR-I and Smad7. Of 30 cases of psoriasis in active stage, 28, 24 and 26 cases were positive for USP15, TβR-I and Smad7 staining, respectively. The positive rates of USP15 and Smad7 were significantly higher in psoriasis specimens than in normal skin specimens (44.1%±26.0% vs. 6.1%±6.6%, 47.2%±27.1% vs. 6.6%±7.1%), and positive rate of TβR-I (20.3%±22.2%) in psoriasis was lower than that in normal skin specimens (46.7%±18.2%). There was a significant positive correlation between USP15 and Smad7 expression, and significant negative correlations between USP15 and TβR-expression, an I d between TβR- and Smad7 expression I in psoriasis. After transfection of USP15 siRNA in Hacat cells, the expression of TβR-mRNA was up I -regulated and that of Smad7 was down-regulated. It is concluded that USP15 may play a role in the pathogenesis of psoriasis through regulating the TβR-I/Smad7 pathway and there may be other cell signaling pathways interacting with USP15 to take part in the development of psoriasis.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Psoriasis / Skin / Immunohistochemistry / Signal Transduction / Gene Expression / Keratinocytes / Cell Line / Protein Serine-Threonine Kinases / Receptors, Transforming Growth Factor beta / Reverse Transcriptase Polymerase Chain Reaction Limits: Adult / Female / Humans / Male Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Psoriasis / Skin / Immunohistochemistry / Signal Transduction / Gene Expression / Keratinocytes / Cell Line / Protein Serine-Threonine Kinases / Receptors, Transforming Growth Factor beta / Reverse Transcriptase Polymerase Chain Reaction Limits: Adult / Female / Humans / Male Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2014 Type: Article