Screening and identification of receptor reacting with nucleocapsid protein of duck enteritis virus / 病毒学报
Chinese Journal of Virology
;
(6): 63-66, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-354770
ABSTRACT
To clarify the pathogenesis of Duck enteritis virus (DEV), the cDNA library of duck's liver infected by DEV and a bait plasmid containing DEV nucleocapsid protein (NP) gene were constructed, then the receptor was screened from the cDNA library plasmid by the yeast two-hybrid system and verified by GST pull-down test. The results showed that the capacity of the primary cDNA library was 1 x 106 CFU with insertion size from 0.5 to 1 kb, and the bait plasmid of pGBKT7-NP showed no self-activation. The receptor reacting with DEV NP in duck liver was initially confirmed as the protein kinase C inhibitor (PKCI). These results provide new clues for further investigation on pathogenesis of DEV.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Receptors, Virus
/
Virology
/
Virulence
/
Gene Library
/
Alphaherpesvirinae
/
Nucleocapsid Proteins
/
Two-Hybrid System Techniques
/
Ducks
/
Genetics
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2012
Type:
Article
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