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Screening and identification of receptor reacting with nucleocapsid protein of duck enteritis virus / 病毒学报
Chinese Journal of Virology ; (6): 63-66, 2012.
Article in Chinese | WPRIM | ID: wpr-354770
ABSTRACT
To clarify the pathogenesis of Duck enteritis virus (DEV), the cDNA library of duck's liver infected by DEV and a bait plasmid containing DEV nucleocapsid protein (NP) gene were constructed, then the receptor was screened from the cDNA library plasmid by the yeast two-hybrid system and verified by GST pull-down test. The results showed that the capacity of the primary cDNA library was 1 x 106 CFU with insertion size from 0.5 to 1 kb, and the bait plasmid of pGBKT7-NP showed no self-activation. The receptor reacting with DEV NP in duck liver was initially confirmed as the protein kinase C inhibitor (PKCI). These results provide new clues for further investigation on pathogenesis of DEV.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Receptors, Virus / Virology / Virulence / Gene Library / Alphaherpesvirinae / Nucleocapsid Proteins / Two-Hybrid System Techniques / Ducks / Genetics Type of study: Diagnostic study / Prognostic study / Screening study Limits: Animals Language: Chinese Journal: Chinese Journal of Virology Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Receptors, Virus / Virology / Virulence / Gene Library / Alphaherpesvirinae / Nucleocapsid Proteins / Two-Hybrid System Techniques / Ducks / Genetics Type of study: Diagnostic study / Prognostic study / Screening study Limits: Animals Language: Chinese Journal: Chinese Journal of Virology Year: 2012 Type: Article