Influence of cytochrome C on apoptosis induced by daunorubicine in acute myeloid leukemia (AML) cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 282-285, 2005.
Article
in Zh
| WPRIM
| ID: wpr-356576
Responsible library:
WPRO
ABSTRACT
The purpose was to study the responses of AML cell treated with cytochrome C and to explore the influence of cytochrome C on apoptosis of AML cell induced by daunorudicine (DNR). The differentiation of AML cell was detected by Wright-Giemsa staining and NBT test, the apoptosis of AML cell was assayed by flow cytometry and fluorescence microscopy. The results showed as follows: (1) different concentrations of cytochrome C could induce different effects on AML cells. Concentration of cytochrome C for differentiation was 10 microl/ml, for apoptosis was 20 microl/ml, and for necrosis was 40 microl/ml. (2) the apoptosis of AML cells decreased with the administration of cytochrome C in 10.0 microg/ml before treating AML cells with DNR (P < 0.01), but no change was shown with the administration of cytochrome C in 20.0 microg/ml (P > 0.05). (3) in reverse sequence, administrating of cytochrome C in 10 microl/ml and 20 microl/ml after treating AML cells with DNR, two different concentrations of cytochrome C could increase the apoptosis of AML cells (P < 0.01). It is suggested that cytochrome C may probably affect the apoptosis of AML cells induced by DNR.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Tumor Cells, Cultured
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Leukemia, Myeloid, Acute
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Daunorubicin
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Apoptosis
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Cytochromes c
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Dose-Response Relationship, Drug
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Drug Synergism
/
Flow Cytometry
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article