Effects of different dose microwave radiation on protein components of cultured rabbit lens / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 208-210, 2007.
Article
in Zh
| WPRIM
| ID: wpr-357554
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of different dose microwave radiation on protein components of cultured rabbit lens, and analyze the mechanisms of lens injury caused by microwave radiation.</p><p><b>METHODS</b>Cultured rabbit lens were exposed to microwave radiation with frequency of 2450 MHz and power density of 0.25, 0.50, 1.00, 2.00, 5.00 mW/cm(2) for 8 hours in vitro. The transparency of lens was observed. Changes of protein concentration were detected after different lens protein components were extracted, including water-soluble protein (WSP), urea soluble protein (USP), alkali soluble protein (ASP) and sonicated protein (SP). The influence of microwave radiation on WSP was analyzed using SDS-PAGE electrophoresis and coomassie-blue staining.</p><p><b>RESULTS</b>Transparency of lens decreased after radiation. There was obvious opacification of lens cortex after 5.00 mW/cm(2) microwave radiation for 8 hours. After 1.00, 2.00 and 5.00 mW/cm(2) radiation, the percentage of WSP decreased while USP increased obviously. There was no change of ASP. The percentage of SP decreased when the power of microwave was 5.00 mW/cm(2). The low molecular weight protein of WSP decreased while high molecular weight protein increased after microwave radiation.</p><p><b>CONCLUSION</b>Microwave radiation higher than 1.00 mW/cm(2) can affect the proportion of WSP and USP in cultured rabbit lens, and cause changes of lens transparency and refractive power, which leads to lens opacity.</p>
Full text:
1
Index:
WPRIM
Main subject:
Radiation Effects
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In Vitro Techniques
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Proteins
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Lens, Crystalline
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Metabolism
/
Microwaves
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2007
Type:
Article