Relationship between single nucleotide polymorphisms in the promoter of COX-2 gene and hereditariness to NAFLD / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 773-777, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-360843
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between COX-2 gene and hereditariness to Nonalcoholic fatty liver disease by detecting single nucleotide polymorphisms in the promoter of COX-2 gene.</p><p><b>METHODS</b>Genotypes of 200 case patients with NAFLD and 206 control subjects were examined by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). The DNA samples were extracted from the peripheral blood of all subjects.</p><p><b>RESULTS</b>Two SNPs, -1195G more than A and -765 G more than C, were identified with frequencies of variant alleles 54% and 5% in patients with NAFLD and 48% and 2% in control, respectively. A case-control analysis revealed a 1.13-fold (95% CI = 1.01-2.46) and a 2.35-fold (95% CI = 1.17-3.65) excess risk of developing NAFLD for -1195AA or -765CG genotype carriers compared with noncarriers. Compared with G-1195-G-765 containing haplotype, a greater risk of developing NAFLD was observed for A-1195-G-765 (OR =1.42; 95% CI =1.11-1.63) and A-1195-C-765 (OR = 4.24; 95% CI =1.72-14.22) containing haplotypes. A greater risk of developing NAFLD was observed for A-1195 and C-765 containing haplotype compared with other haplotype, suggesting an interaction between the -1195A and -765C in the context of haplotype.</p><p><b>CONCLUSIONS</b>These findings suggest that genetic variants in the COX-2 promoter may play an important role in mediating susceptibility to developing NAFLD in a Chinese population. -1195G more than A and -765G more than C in promoter region of Cyclooxygenase-2 gene, whose single nucleotide polymorphisms are related with development of NAFLD, are the significance factors of the susceptibility of NAFLD.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Haplotypes
/
Case-Control Studies
/
Promoter Regions, Genetic
/
Genetic Predisposition to Disease
/
Polymorphism, Single Nucleotide
/
Alleles
/
Cyclooxygenase 2
/
Fatty Liver
/
Non-alcoholic Fatty Liver Disease
/
Genetics
Type of study:
Observational study
/
Prognostic study
/
Risk factors
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2010
Type:
Article
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