Experimental Study of Microvascular Anastomosis Using GRF Glue / 日本心臓血管外科学会雑誌

ABSTRACT

The GRF glue consists of mixture of gelatine and resorcine. The mixture is hardened by the addition of medical formaldehyde. Resorcine is diphenole which reacts with formaldehyde, creating tridimentional network. We performed microvascular anastomosis of abdominal aorta of the rat using GRF glue and the histologial study by the light microscope and the scanning electron microscope. Re-endoterization began from two days after anastomosis and completed at ten days to two weeks after anastomosis. From scanning electron microscopic study the invasion of macrophages and platelets in the case of anastomosis using GRF glue was less than in the case of manual or laser anastomosis. We revealed that GRF glue is very useful for bonding of micro vessels.