Your browser doesn't support javascript.
loading
Effects of alkaloid sinomenine on expression levels of nuclear factor of activated T cells and interferon-gamma in CD4+ T lymphocytes of human periphery blood in vitro / 中华器官移植杂志
Chinese Journal of Organ Transplantation ; (12): 496-499, 2010.
Article in Chinese | WPRIM | ID: wpr-387699
ABSTRACT
Objective To study the immunosuppressive mechanism of alkaloid sinomenine (SIN) by observing the effects of SIN on the proliferation and intracellular protein expression levels of nuclear factor of activated T cells (NF-AT) and interferon-gamma (IFN-γ) in CD4+ T lymphocytes of human periphery blood. Methods CD4+ T lymphocytes were isolated from PBMC suspensions with immunomagnetic beads and divided into five groups to culture. (1) Negative control group no medicine was added to cell culture medium; (2) Positive control group CsA solution (final concentration 50ng/ml) was added to cell culture media; (3) Low-concentration SIN group (L-SIN) low-concentration SIN solution (final concentration 10 μmol/L) was added to cell culture media; (4) Middle-concentration SIN group (M-SIN) middle-concentration SIN solution (final concentration 200 μmol/L) was added to cell culture media; (5) High-concentration SIN group (H-SIN) high-concentration SIN solution (final concentration 1000 tmol/L) was added to cell culture media. The proliferations of CD4+ T lymphocytes were observed. Western blotting was performed to detect the protein expression levels of NF-AT. FCM was used to determine the levels of IFN-γ. Results Compared with negative control group, the cell proliferation was significantly inhibited in H- and M-SIN groups (P<0. 01 ). SIN concentration-dependently inhibited the protein expression levels of NF-AT and IFN-γ in CD4+ T lymphocytes of human periphery blood (P<0.01). The protein expression levels of NF-AT and IFN-γ were lowest in positive control group. There was a close negative correlation between intracellular levels of NF-AT and cell proliferation inhibition ratio in CD4+ T lymphocytes of human periphery blood (rs = - 0. 969, P = 0. 000). Conclusion SIN can inhibit the protein expression of NF-AT and IFN-γ in CD4+ T lymphocytes of human periphery blood probably by decreasing protein levels of NF-AT to inhibit the activity and proliferation of CD4+ T lymphocytes.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Organ Transplantation Year: 2010 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Organ Transplantation Year: 2010 Type: Article