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Effect of heme oxygenase-1 over-expression on the survival of rats after reduced-size liver transplantation / 中国组织工程研究
Chinese Journal of Tissue Engineering Research ; (53): 10401-10406, 2008.
Article in Chinese | WPRIM | ID: wpr-406792
ABSTRACT

BACKGROUND:

Heme oxygenase-1 (HO-1) plays an important role on preventing tissues and organs from oxidant stress injury, which remains currently one of the most active areas of investigation.

OBJECTIVE:

To study HO-1 over-expression on the survival and fiver function of rats after reduced-size liver transplantation by constructing recombinant adenovims Ad5-HO-1.DESIGN, TIME AND

SETTING:

Randomized controlled animal study was performed in the Institute of Pediatrics, Children Hospital Affiliated to Chongqing Medical University from September 2003 to March 2005. MATERIALS Seventy-four SD rats were used to establish in situ reduced-sized liver transplantation models.

METHODS:

Recombinant adenoviral vector encoding Ha-1 gene (AdS-HO-1) was generatred by molecular biology method, wluch was administered to donors via voln at 48 hours before transplantation. All rats were randomly divided into control group(n=12),saline group(n=12), cobalt protoporphyrin (CoPP)group(n=13),Ad5-HO-1 group(n=13),Ad5-green fluorescent protein(GFP)group(n=12),and Ad5-HO-1+zinc protoporphyrin (ZnPP)group (n=12).Livers of donor wefe harvested and stored for 24 hours at 4℃ in HTK solution. Before it is implantedinto recipient. MA IN OUTCOME

MEASURES:

Survival rate and liver function; portal vein blood flow monitored 2 hours after transplantation by Color Doppler How Imaging; pathological changes of transplanted liver tissue observed by HE staining;HO-1 activity, tumor necrosis factor-alpha(TNF-α),Bcl-2 and Bax expression detected by immunohistoehemical staining; changes of HO-1,TNF-α,bcl-2 and bax mRNA detected by molecular viewpoint.

RESULTS:

Survival rate in the Ad5-HO-1 group was significantly higher than that in the saline group at al,7,and 21 days after liver transplantation(P<0.05).Glutamic pyruvic transaminase decreased in the Ad5-HO-1 group as compared to that in the saline,Ad5-GFP,and Ad5-HO-1+ZnPP groups(P<0.05);portal venous blood flow significantly increased 2 hours after transplantation(P<0.05);HO-1 activity also significantly increased(P<0.05).RT-PCR and immunohistochemical staining showed that HO-1 and bcl-2 expressions increased(P<0.05),but bax and TNF-αexpressions decreased(P<0.05).

CONCLUSION:

Ad5-HO-1 significantly induces high expression of HO-1 increases portal venous blood flow within 2 hours alter liver transplantation, and promotes liver functional recovery so as to prolong survival time of rats after liver transplantion.
Full text: Available Index: WPRIM (Western Pacific) Type of study: Controlled clinical trial / Prognostic study Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Controlled clinical trial / Prognostic study Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2008 Type: Article