Determination of the Contents of Puerarin in Infant Fuxieling Effervescent Granules With HPLC / 中国药房
China Pharmacy
;
(12): 238-239, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-410715
ABSTRACT
OBJECTIVE:
To establish a method for determination of the contents of puerarin in infant Fuxieling effervescent granules with HPLC.METHODS:
The puerarin was extracted by ultrasonics, using methanol as solvate.The stratographic column was Shim - Pack C1s with acetonitrile - water( 10 90) as mobile phase. The flow rate was 2.0ml/min and the detecting wavelenghth was 250nm.The temperature of column was 25℃ .RESULTS:
The resolution between the puerarin and the adjacent peaks was >1.5 .The number of theoretical plates was 18 550 .The standard curve was linear in the range of 0.964μg~7. 712μg .The average recovery was 100.2% (RSD = 1.76%, n = 6).CONCLUSION:
The method is simple, rapid and accurate. It can be used for quality control of the preparation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Type:
Article
Similar
MEDLINE
...
LILACS
LIS