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Effect of angiotensin 1-7 on human renal proximal tubular epithelial cell transdifferentiation induced by high glucose and its mechanism / 中华肾脏病杂志
Chinese Journal of Nephrology ; (12): 903-906, 2011.
Article in Zh | WPRIM | ID: wpr-428209
Responsible library: WPRO
ABSTRACT
Objective To investigate the effect of angiotensin 1-7(Ang 1-7) on renal proximal tubular epithelial cell(HK-2) transdifferentiation induced by high glucose.Methods All the raised HK-2 cells were divided into 5 groups: normal control group,high glucose group,high glucose with Ang1-7 group,high glucose with Ang1-7 and A779 group,high glucose with pioglitazone group.Expression of peroxisome proliferator activated receptor-γ(PPAR-γ) and α-smooth muscle actin(α-SMA) was detected by Western blotting,real-time PCR and immunofluorescence.Results The levels of PPAR-γ protein and mRNA in HK-2 cells were significantly increased after treatment with high glucose and Ang 1-7.Expression of α-SMA protein and mRNA was inhibited remarkably after treatment with high glucose and Ang 1-7.These effects of Ang 1-7 on HK-2 cells could be reversed by Mas receptor antagonist A779.Conclusion Ang 1-7 inhibits high glucose-induced expression of o-SMA in HK-2 cells,which is in part through the Mas.
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Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Nephrology Year: 2011 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Nephrology Year: 2011 Type: Article