Preparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration
Clinical and Experimental Vaccine Research
;
: 61-66, 2017.
Article
in English
| WPRIM
| ID: wpr-43943
ABSTRACT
PURPOSE:
Nitrocellulose membrane–based filtration system (NCFS) is widely used for protein concentration. In this study, we applied NCFS for production of virus-like particle (VLP) as a vaccine candidate and evaluated yield property and immunogenicity. MATERIALS ANDMETHODS:
Influenza VLPs were generated by baculovirus-insect cell protein expression system. NCFS and sucrose gradient ultracentrifugation were used for purification of VLP. Immunogenicity of VLP was evaluated by animal experiment.RESULTS:
Influenza VLPs expressing hemagglutinin (HA) and neuraminidase proteins derived from highly pathogenic influenza virus (H5N8) were effectively produced and purified by NCFS. HA activity of VLP which correlated with antigenicity was well conserved during multiple purification steps. This NCFS based purified VLPs induced influenza virus–specific antibody responses.CONCLUSION:
Our results indicate that the influenza VLP vaccine could be prepared by NCFS without loss of immunogenicity and elicit antigen-specific immune responses.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Orthomyxoviridae
/
Sucrose
/
Ultracentrifugation
/
Vaccines
/
Baculoviridae
/
Collodion
/
Animal Experimentation
/
Influenza, Human
/
Filtration
/
Hemagglutinins
Language:
English
Journal:
Clinical and Experimental Vaccine Research
Year:
2017
Type:
Article
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