Nested PCR definition of species composition of malaria parasites in Thanh commune, Huong Hoa district of Quang Tri province

ABSTRACT

Nested polymerase chain reaction (nested PCR) was applied to idennify species composition of four human malaria parasite species in Thanh commune, Huong Hoa district of Quang Tri province. The pair of primers specific to each species of P.falciparum, P.vivax, P.malariae and P.ovale were used. AND fragments specific to each species was 18ssr-RNA)[5], [10]. The analysis of 152 blood samples showed only two species of malaria parasites of P.falciparum (71%) and P.vivax (29%) present in the study area. Single P.falciparum infection rate was 65.8%, single P.vivax infection rate was 16.4% and the double infection of P.falciparum and P.vivax was 17.8%