Direct activation of expression vector withβ-casein gene promoter using engineered TALE transcription factors in mouse fibroblasts / 中国实验动物学报
Acta Laboratorium Animalis Scientia Sinica
; (6): 13-16, 2014.
Article
in Zh
| WPRIM
| ID: wpr-459075
Responsible library:
WPRO
ABSTRACT
Objective TALE-TFs were adopted to provide a new way in detection of the expression result ofβ-ca-sein gene promoter-interesting gene expression cassettes in mouse fibroblasts.Methods TALE-TFs of eukaryotic expres-sion plasmid and expression cassette withβ-casein gene promoter and red fluorescent protein reporter gene were co-nucleo-fected into mouse fibroblasts by Amaxa nucleofector.Results and Conclusion β-casein gene promoter was activated by artificial TALE-TFs in the mouse fibroblasts.The way is a new expression verification system instead of mammary epithelial cells with fibroblasts.
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WPRIM
Language:
Zh
Journal:
Acta Laboratorium Animalis Scientia Sinica
Year:
2014
Type:
Article