The cloning and expression of nucleoproteins (NP) of Ebola virus / 军事医学
Military Medical Sciences
; (12): 659-662, 2014.
Article
in Zh
| WPRIM
| ID: wpr-459473
Responsible library:
WPRO
ABSTRACT
Objective To provide the candidate antigens for immunological diagnosis by analyzing the expression of nu -cleoprotein ( NP) of Ebola virus. Methods BioSun software was used to predict the NP epitopes. The bridging-PCR was used to synthesize the NP gene. The pBVIL1 vector was used to clone and express the NP gene. Results The 360-739 aa of NP was confirmed to be the dominant antigen by BioSun software. The recombinant NP dominant antigen was expressed in E.coli with molecular weight of 58 ×103.The specificity of ELISA based on recombinant NP was 99.24% (130/131) in negative samples. Conclusions The dominant NP antigen can be potentially used for developing Ebola virus diagnostic reagent.
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WPRIM
Language:
Zh
Journal:
Military Medical Sciences
Year:
2014
Type:
Article