Your browser doesn't support javascript.
loading
The protective role of mammalian target of rapamycin in the injury of podosytes induced by adriamycin / 国际儿科学杂志
Article in Zh | WPRIM | ID: wpr-474631
Responsible library: WPRO
ABSTRACT
Objective To explore the role of mammalian target of rapamycin (mTOR) complex in the adriamycin induced the podecytes.Methods 1 μmol/L adriamycin (ADM) was used to induce mice podocytes for 24 hours and detected the apoptosis ratio,podosytes damage markers (Desmin) and the related expression of mTOR,then the role of mTOR was analysized in ADM induced podosytes.Results 1 umol/L ADM induced mice podosytes for 24 hours can make the podocytes damage.Compared with control group,podosytes activity declined(t =28.68,P < 0.001),apoptosis rate increased both early (t =10.24,P =10.24) and late (t =4.26,P =4.26),and Desmin expression increased(t =6.16,P <0.001).mTOR expression decreased in ADM group.Compared with control group,both mTOR and phosphorylation mTOR expression decreased (t =3.57,P =3.57).mTOR downstream target molecular S6K1 and 4ebpl mRNA expression decreased(t =18.28,P < 0.001).Conclusion ADM can make podosytes damage and the mechanism of ADM induced podosytes damage may be related to the decreasing mTOR and the low activity of mTOR.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: International Journal of Pediatrics Year: 2014 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: International Journal of Pediatrics Year: 2014 Type: Article