The correlation between the soluble triggering receptor expressed on myeloid cells-1 and the janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway in rats with abdominal sepsis / 中华急诊医学杂志

ABSTRACT

Objective To investigate the correlation between the soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) and the janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway in rats with abdominal sepsis.Methods Using a sepsis model produced by cecal ligation and puncture (CLP),Wistar rats were randomly (random number) divided into normal control group (n =6),sham-operated group (n =24,) CLP group (n =48),AG490 (JAK2 inhibitor) treatment group (n =48) and rapamycin (RPM,STAT3 inhibitor) treatment group (n =48).At different intervals,the rats in each group were sacrificed,then the peripheral blood cells were harvested to detect the percent of CD4 + CD25 + Treg cells in CD4 + cells by flow cytometry.Meanwhile,mRNA of sTREM-1 from the tissue samples of the liver was also measured by real-time reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with the normal control group and the sham-operated group,expression of sTREM-1 in the CLP group was significantly higher,and it showed a gradually increased tendency over time.At 6 and 24 hours,there were no significant differences in the expressions of sTREM-1 between the AG490 group (1.572 ± 0.051,2.063 ± 0.025,respectively) and the CLP group (1.592 ±0.036,2.082 ±0.021,respectively).But the expression of sTREM-lin the AG490 group (2.522 ± 0.083,3.153 ± 0.021,respectively) was lower than those in the CLP group (2.592 ±0.055,3.204 ±0.013,respectively) during 48 and 72 hours.In addition,there was no significant difference in the expression of sTREM-1 between the RPM group (1.581 ± 0.017) and the CLP group (1.592 ±0.036) at 6 hours.And the expression of sTREM-1in the RPM group (1.486 ±0.019,1.263 0.011,1.115 ± 0.022,respectively) was significantly lower than those in the CLP group (2.082 ± 0.021,2.592 ±0.055,3.204 ± 0.013,respectively) during 24 to 72 hours.Conclusions These data proved that blocking JAK/STAT pathway could inhibit the expression of sTREM-1 and decelarate the progress of inflammatory reaction in rats with abdominal sepsis.