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Establishment of chemiluminescence immunoassay for AngiotensinΙ / 中国免疫学杂志
Chinese Journal of Immunology ; (12): 838-841, 2016.
Article in Zh | WPRIM | ID: wpr-490239
Responsible library: WPRO
ABSTRACT
Objective:The chemiluminescence immunoassay for AngiotensinⅠ ( AngⅠ ) was developed by the competition method. The renin activity was calculated by the determination of Ang Ⅰ. Methods: AngⅠ antigen was labeled with biotin and fluorescein labeled AngⅠantibody,and anti fluorescein antibody was used to coated with the microporous plate . Results:The standard range of the method was 100-7 800 pg/ml. The assay sensitivity was 24. 3 pg/ml. The intra- and inter-assay coefficients of variance were 5. 6%-8. 7% and 6. 8%-10. 4% respectively. Analytical recovery was 95. 4%-105. 3%. The correlation coefficients between measured and expected values were 0. 999 after serial diluted. Compared with radioimmunoassay kit,the correlative equation was y=0. 95x+235. 70,correlation coefficient was 0. 973. Coated microporous plate and biotin labeled Ang Ⅰ antigen,and fluorescein labeled Ang Ⅰantibody at 37℃ for a week with good stability. Conclusion: The results of the method were in accord with the basic requirements of immunoassay.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Immunology Year: 2016 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Immunology Year: 2016 Type: Article