Biological characteristics of bone marrow mesenchymal stem cells and application in skin defect repair / 中国组织工程研究

ABSTRACT

BACKGROUND:An appropriate biomaterial can be directly combined with autologous or al ogeneic skin cel s to construct tissue-engineered skin, which can accelerate skin repair after transplantation onto the skin wounds. It is a good idea to solve the deficiency in skin sources. OBJECTIVE:To study the biological characteristics of the complex with bone marrow mesenchymal stem cel s and to investigate the application of this complex in skin defect repair. METHODS:Twenty-eight Sprague-Dawley rats, SPF grade, were randomized into two groups (n=14 per group). By adjustment of temperature, time, pressure and area of NC perm instrument, scald models were made in rats. Rats in the treatment group were given the repair using composite bone marrow mesenchymal stem cel s, while those in the control group were given vaseline gauze repair. Repair effects were compared between two groups. RESULTS AND CONCLUSION:(1) After 72 hours of culture, the composite bone marrow mesenchymal stem cel s smal with round shape distributed dispersedly. After 5 days of culture, the cel s began to stretch and the cel morphology became unstable. After three passages, the cel morphology became stable. The results of antigen identification showed the expression of CD44 and CD29 but the low expression of CD45 and CD34 in bone marrow mesenchymal stem cel s. (2) Twenty-eight days after repair, there was no obvious scar on the wound surface of the treatment group, but a little shrinkage and obvious scar stil existed in the control group. Moreover, in the control group, the epidermal layer of the skin was relatively thick, and the connection between the basal layer and the dermis was unsatisfactory. In the treatment group, obvious epidermal cel stratification, neat arrangement, and tight connection between the epidermis and dermis were observed. In summary, bone marrow mesenchymal stem cel s are a special class of cel s that have pluripotent ability and are more readily available. These cel s are the preferred target cel s for skin defect repair to promote early healing of the skin and improve blood circulation defect site, which are confirmed to have high clinical value.