Determination of the Related Substances in Anastrozole by Improving HPLC / 中国药房
China Pharmacy
; (12): 1275-1278, 2017.
Article
in Zh
| WPRIM
| ID: wpr-515160
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:To establish a method for the determination of related substances in anastrozole. METHODS:HPLC was performed on the column of Welch Materials XB C18 with mobile phase of acetonitrile-water(35:65,V/V)at a flow rate of 1.0 mL/min,detection wavelength was 210 nm,column temperature of 25 ℃,and the injection volume was 20 μL. RESULTS:Only one impurity(impurity A)was detected in anastrozole,the linear range was 0.1-1.6 μg/mL(r=0.9996);limit of quantitation was 0.1 μg/mL,limit of detection was 0.02 μg/mL;RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 98.95%-105.29%(RSD%=1.78%,n=9). CONCLUSIONS:The method is simple,accurate,and can be used for the determination of related substances in anastrozole.
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WPRIM
Language:
Zh
Journal:
China Pharmacy
Year:
2017
Type:
Article