Plasma Total Homocysteine and Influential Factors in Hemodialysis Patients / 中国医师杂志

ABSTRACT

Objective In order to investigate plasma total homocysteine(tHCY) level,its influential factors,and the relationship between total homocysteine(tHCY)and N 5,N 10 -methylenetetrahydrofolate reductase(MTHFR) gene in hemodialysis patients.Method Pre-and posthemodialysis plasma tHCY and MTHFR genotype were measured by high performance liquid chromatography(HPLC) method and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method respectively,and dialysis related indexes were detected simultaneously.Results Plasma tHCY in hemodialysis patients was(28 4?5 8)?mol/L,and it was significantly higher than normal control(P