The effect of IGF- I receptor monoclonal antibody on human colorectal cancer cell lines / 中国普通外科杂志

ABSTRACT

Objective To explore the expression of insulin-like growth factors and their receptor (IGF- I R) in a human colorectal cancer cell line HT-29, and the effect of two IGF- I R monoclonal antibodies on the biological behavior of HT-29 cell lines. Methods Immunohistochemistry was used to test the expression of IGF- I R in HT-29 cell lines. MTT assay was used to determine the anti-proliferation effects of IGF- I R monoclonal antibody (McAb). Apoptosis percentage was estimated by flow cytometry ( FCM). Results IGF- I R was expressed in the membranes of HT-29 cell lines. McAb of IGH- I R can inhibit the proliferation of HT-29 cell lines in a dose-dependent manner. The apoptosis rate of HT-29 cells treated by IGF- I R McAb was higher than that of control group (P