Determination of Flavonoids in Different Parts and Different Harvesting Seasons of Hypericum japonicum / 中国药房

ABSTRACT

OBJECTIVE: To determine the contents of flavonoids in different part and different harvesting seasons of Hypericum japonicum.METHODS: The total content of flavonoids in H.japonicum was determined by colorimetry and the contents of quercetin,rutin and isorhamnetin in H.japonicum were determined by HPLC.RESULTS: The H.japonicum harvested in different seasons showed no significant difference in terms of the contents of total flavonoids.H.japonicum from Guangxi province contained no isorhamnetin but which was rich in the contents of quercetin and rutin.In H.japonicum,the content of total flavonoids was rich in the seeds and leaves but poor in the roots,and hardly had isorhamnetin,quercetin and rutin been detected in the roots.CONCLUSION:H.japonicum harvested in the spring season is low and small in size with low-yield;therefore,fall is its optimal harvesting season.Furthermore,the specification in Chinese Pharmacopeia that the medicinal part of H.japonicum is the entire herb has been proved to be scientific.