Determination of S-Omeprazole Sodium and the Related Substances by RP-HPLC / 中国药房
China Pharmacy
;
(12)2005.
Article
in Chinese
| WPRIM
| ID: wpr-532675
ABSTRACT
OBJECTIVE:
To establish an RP-HPLC method for content determination of s-omeprazole sodium and its related substances.METHODS:
The separation of s-omeprazole sodium and the related substances was carried out on a Phenomenex Luna C18 column,the mobile phase consisted of methanol-0.033 mol?L-1 ammonium dihydrogen phosphate-triethylamine (58∶41.8∶0.2,adjusted to pH 7.0 by phosphate acid).The detection wavelength was 302 nm,the flow rate was 1.0 mL?min-1,the column temperature was 25 ℃,and the sample size was 20 ?L.RESULTS:
The linear range of omeprazole sodium was 10~500 mg?L-1 (r=0.999 7).The average recovery rate was 100.27% (RSD=0.74%).The average content of the related substances in samples was 0.42%.CONCLUSION:
This method is simple,accurate,specific and applicable for content determination of s-omeprazole sodium and its related substances.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS