Determination of the Content of Vitamin A Palmitate and Its Related Substances by HPLC / 中国药房
China Pharmacy
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-532798
ABSTRACT
OBJECTIVE:
To establishment the method for the determination of content of vitamin A palmitate(VAP) and its related substances by HPLC.METHODS:
The HPLC conditions were consisted of Phenomenex Luna C18 column with a mobile phase of a mixture of acetonitrile-isopropanol (90∶10) ,the detection wavelength of 328 nm,the column temperature of 30 ℃ and the flow rate of 1.0 mL?min-1.RESULTS:
VAP was completely separated from impurities,the linearity range was 90~400 mg?L-1(r=0.999 2). The average recovery rate was 99.60% (RSD=1.32%). The average content of the related substances were lower than 2.53% .CONCLUSION:
This accurate and reliable HPLC method is applicable for the quality control of VAP.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2007
Type:
Article
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